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Fig. 1 FGF/ERK signalling antagonises endoderm formation. (A-F) Expression of sox17 at 50% epiboly (A,B) or 80% epiboly (C-F). Injection of ERK2* RNA at 50 ng/µl (B), Fgf8 ligands RNA at 10 ng/µl (C) or RNA encoding constitutively activated form of Ras at 5 ng/µl (D) at the one-cell stage inhibits endoderm specification. (E,F) The triple morpholino knockdown of Fgf8, Fgf17b and Fgf24 causes overproduction of endoderm precursors. (A,B) Animal pole views; (C,D) dorsal views; (E,F) lateral views.
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