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Figure Caption
Fig. 4 Mapping of the transcriptional initiation site of the human βB1-crystallin gene in a stable line of transgenic zebrafish. A: The -90/+61-bp human βB1-crystallin promoter-integrated stable line of transgenic zebrafish was utilized to define the transcriptional initiation site. 5'RACE was performed, and the PCR product showed a major band (arrow indicated). M: 100-bp DNA marker. B: The precise initiation site was revealed by sequencing, beginning with an A base.
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