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Figure Caption
Fig. 5 Electrophoretic mobility shift assay (EMSA) demonstrating a cis-regulatory element in the proximal promoter region. A: Oligonucleotides (1, 2, 3) harboring the -98/-19-bp proximal promoter region were tested with zebrafish embryo extract. B,C: The specificity of the DNA-protein complex was further analyzed using zebrafish embryos and mouse lens nuclear extract in the presence of non-radioactive competitive probes. The probes and specific DNA-protein complexes are, respectively, indicated by the letters P and C. The precise sequences of these oligonucleotides are marked in Figure 6A.
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