ZFIN Image: Hou et al., 2006, Fig. 7

Image

Figure Caption

Fig. 7 Maf binding cis-element is crucial for the upstream promoter activity of the human βB1-crystallin gene. Top: A point mutation was introduced in the single Maf binding site of the human $beta;B1-crystallin promoter. The mutated and wild-type upstream promoter -2,966/+61-bp fragments were analyzed in zebrafish by transient transgenic assay. Mutation of the Maf binding site severely impeded the human βB1-crystallin promoter function as reflected by the decrease in percentage of injected embryos that express EGFP in the lens. A-D: With the wild-type construct, intensive expression of EGFP is shown in the whole lens of most embryos (A), and in a few embryos with mosaic patterns (B-D). E-H: Embryos injected with the mutated construct show highly mosaic patterns and the reporter gene, EGFP, is faintly expressed.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.