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Figure Caption
Fig. 2 Promoter activity of the human βB1-crystallin gene demonstrated by expression of EGFP (enhanced green fluorescent protein) in transient transgenic experiments in zebrafish. A-E: Constructs of serially deleted promoter fragments shown in Figure 1 were analyzed in zebrafish embryos. The reporter gene, EGFP, was specifically observed to be expressed in the whole lens in most embryos. F: Some F0 transient transgenic fish were raised to adulthood, and showed robust expression of EGFP in the lens (F, here with the construct of the -90/+61-bp promoter).
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