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Fig. 3 The brain does not extend properly in kto mutants. Wild-type (A and C-E) and kto mutant (B and F). (A-D) In situ hybridization with flh; a gap (arrowhead) is seen in expression between telencephalon and diencephalon (epiphysis) in wild-type but not in kto embryos (B) or embryos injected with 2 ng of trap230 MO (D). (E and F) wnt1 expression in dorsal midbrain and midhindbrain boundary. Arrow shows reduced midbrain size in kto mutants. di, diencephalon; m, midbrain; mhb, mid-hindbrain boundary; te, telencephalon.
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