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Fig. 5 Regulation of atf7a expression by transcription factors Ntl, Spr2, and Flh. A,E: Control embryos injected with green fluorescent protein (GFP) mRNA. B,D,F: Injection with 150 pg of ntl mRNA (B), 100 pg of spr2 mRNA (D), or 100 pg of flh mRNA (F) enhanced atf7a expression. C: Injection with 10 ng of ntl-morpholino inhibited atf7a expression. G,H: The expression of atf7a was not detected in the flhnl mutant embryo (H), whereas it was expressed in the wild-type embryo from the same cross (G). A-D,G,H: The bud stage. E,F: The 90% epiboly stage. All embryos were dorsal views, with the animal pole to the top.