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Fig. 4 Gene expression analysis for genes involved in optic cup formation and outer nuclear layer differentiation. A,B: In situ hybridization with mRNA probes of six6 (A) and rx2 (B) at 24 hours postfertilization (hpf). C,D: Dorsal views of embryos from clutches of dazed heterozygous matings were analyzed for the expression of (C) shh or (D) rx2 at 48 hpf. Embryos (n ≥ 20) from clutches of dazed heterozygous matings showed no difference in expression patterns of any genes tested.
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