|
Fig. 3 eve1 expression is dependent on Bmp signaling both during and after gastrulation. Embryos were heatshocked in an air incubator at the indicated stages, and the embryos containing the transgene were sorted by GFP fluorescence. (A,B) Vegetal views of embryos heatshocked at shield stage and assayed for eve1 expression at 70% epiboly. Note the light staining on the left (ventral) side of the transgenic embryo (B) compared with the wild-type sibling (A). (C,D) Posterior views of embryos heatshocked at bud stage and assayed for eve1 expression at the 7-somite stage. Note the darker staining of eve1 in the wild-type embryo (C), with expression extending farther anteriorly than in the transgenic embryo (D). (E,F) Posterior views of embryos heatshocked at the 3-somite stage, then assayed for eve1 expression at the 9-somite stage. Note the darker staining of eve1 in the wild-type embryo (E) than in the transgenic embryo (F). Insets in C-F show lateral views of the tailbud. HS, heatshock.