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Fig. S3 Western blot showing the total amounts of E-Cadherin protein from wild-type and e-cadherin morpholino (MO; 8ng)-injected embryos at bud (10 hpf) and shield (6 hpf) stages. The size of the band recognized corresponds to the predicted molecular weight for E-Cadherin (140 kDa) (Babb and Marrs, 2004). wt, wild type; MO, e-cadherin MO. Two independent samples for each condition with 10 embryos/sample were loaded. As a loading control, the gels were Commassie stained to check for equal amounts of protein in each lane.
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