Gene Trap Construct

Gt(LOXP-GAL4-VP16-FRT,5xUAS:EGFP-FRT-LOXP)

ID

ZDB-GTCONSTRCT-151022-1

Previous Names

GBT-B4 (1)
pDB899 (1)
Gt(:LOXP-GAL4-VP16-FRT,5xUAS:EGFP-FRT-LOXP)
Gt(LOXP-GAL4-VP16-FRT, syUAS:EGFP-FRT-LOXP) (1)
Gt(LOXP-GAL4-VP16-FRT,5xUAS:EGFP-FRT-LOXP)
gt(loxp-gal4-vp16-frt,5xuas:egfp-frt-loxp)

Type

engineered_plasmid

Regulatory Regions

None

Coding Sequences

EGFP (1)
FRT (1)
GAL4 (1)
LOXP (1)
VP16 (1)

[Em λ][Ex λ]

Fpbase:EGFP

Contains Sequences

T2M (1)

Note

This construct contains syUAS, a hybrid synthetic/yeast-based form of UAS.

Plasmid Map

None

Genomic Features

That Utilize Gt(LOXP-GAL4-VP16-FRT,5xUAS:EGFP-FRT-LOXP) Construct

Genomic Feature	Affected Genomic Regions
fcc118Gt	unm_fcc118
fcc143Gt	ubap1
fcc144p1Gt	unm_fcc144p1
fcc144p2Gt	unm_fcc144p2
fcc144p3Gt	unm_fcc144p3
fcc24Gt	ralgds
fcc27Gt	slc2a1a
fcc301Gt	agtpbp1
fcc313Gt	unm_fcc313
fcc316Gt	unm_fcc316
fcc322Gt	unm_fcc322
fcc337p1Gt	unm_fcc337p1
fcc337p2Et	unm_fcc337p2
fcc360p1Gt	unm_fcc360p1
fcc360p2Gt	unm_fcc360p2
fcc360p3Gt	unm_fcc360p3
fcc363p1Gt	exosc3
fcc363p2Gt	unm_fcc363p2
fcc374p1Gt	unm_fcc374p1
fcc374p2Gt	unm_fcc374p2
fcc382p1Gt	unm_fcc382p1
fcc382p2Gt	unm_fcc382p2
fcc403Gt	vps35
fcc413Gt	unm_fcc413
fcc418p1Gt	ric8a
fcc418p2Gt	unm_fcc418p2
fcc420Gt	dnajb14
fcc423p1Gt	unm_fcc423p1
fcc423p2Gt	unm_fcc423p2
fcc436p1Gt	eps15l1b
fcc436p2Gt	unm_fcc436p2
fcc436p3Gt	unm_fcc436p3
fcc443Gt	clybl
fcc458Gt	unm_fcc458
fcc460Gt	unm_fcc460
fcc474p1Gt	unm_fcc474p1
fcc475Gt	unm_fcc475
fcc510Gt	unm_fcc510
fcc516Gt	unm_fcc516
fcc522Gt	unm_fcc522
fcc559Gt	adamts3
fcc5770Gt	unm_fcc5770
fcc618Gt	unm_fcc618
fcc629Gt	eif3c
fcc634Gt	unm_fcc634
fcc652Gt	unm_fcc652
fcc667Gt	abi1a
fcc683Gt	unm_fcc683
fcc688Gt	hnrpkl
fcc711Gt	unm_fcc711
fcc88p1Gt	vps4b
fcc88p2Gt	unm_fcc88p2
tpl50Gt	fli1rs

Transgenics

That Utilize Gt(LOXP-GAL4-VP16-FRT,5xUAS:EGFP-FRT-LOXP) Construct

Fish	Affected Genomic Regions	Phenotype	Gene Expression
abi1a^fcc667Gt/+ (AB)	abi1a
abi1a^fcc667Gt	abi1a		2 figures from Seiler et al., 2015
adamts3^fcc559Gt	adamts3		2 figures from Seiler et al., 2015
agtpbp1^fcc301Gt/+ (AB)	agtpbp1
agtpbp1^{fcc301Gt/fcc301Gt}	agtpbp1		Fig. 4 from Seiler et al., 2015
agtpbp1^fcc301Gt	agtpbp1		2 figures from Seiler et al., 2015
eps15l1b^fcc436p1Gt/+ (AB)	eps15l1b
eps15l1b^{fcc436p1Gt/fcc436p1Gt}	eps15l1b		Fig. 4 from Seiler et al., 2015
eps15l1b^fcc436p1Gt	eps15l1b		3 figures from Seiler et al., 2015
fli1rs^tpl50Gt/+; y1Tg + MO1-etsrp + MO2-etsrp		Fig. 3 from Craig et al., 2015	Fig. 3 from Craig et al., 2015
fli1rs^{tpl50Gt/tpl50Gt}	fli1rs		Fig. 6 from Craig et al., 2015
fli1rs^{tpl50Gt/tpl50Gt} + MO1-etsrp + MO2-etsrp			2 figures from Craig et al., 2015
fli1rs^{tpl50Gt/tpl50Gt}; y1Tg	fli1rs		3 figures from 2 publications
fli1rs^{tpl50Gt/tpl50Gt}; y1Tg + MO1-etsrp + MO2-etsrp		2 figures from Craig et al., 2015	2 figures from Craig et al., 2015
fli1rs^{tpl50Gt/tpl50Gt}; y1Tg + MO2-etsrp		Fig. 6 from Baltrunaite et al., 2017	Fig. 6 from Baltrunaite et al., 2017
hnrpkl^fcc688Gt	hnrpkl		2 figures from Seiler et al., 2015
ralgds^fcc24Gt	ralgds		2 figures from Seiler et al., 2015
ubap1^{fcc143Gt/fcc143Gt}	ubap1		Fig. 4 from Seiler et al., 2015
ubap1^fcc143Gt	ubap1		2 figures from Seiler et al., 2015
unm_fcc337p1^fcc337p1Gt	unm_fcc337p1		Fig. 2 from Seiler et al., 2015
unm_fcc510^fcc510Gt	unm_fcc510		2 figures from Seiler et al., 2015
unm_fcc522^fcc522Gt	unm_fcc522		2 figures from Seiler et al., 2015
vps35^fcc403Gt	vps35		2 figures from Seiler et al., 2015
vps4b^fcc88p1Gt	vps4b		2 figures from Seiler et al., 2015

Sequence Information

Other Construct Pages

None

Citations