FIGURE

Fig. 3

ID

ZDB-FIG-241120-39

Publication

Baril et al., 2024 - The role of ATP-binding Cassette subfamily B member 6 in the inner ear

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Fig. 3

Characterization of zebrafish ABCB6 (Abcb6).

a Alignment of homologous ABCB6 sequences shows zebrafish Abcb6 lacks the N-linked glycosylation site⁸⁹. b Western blot of samples from PNGase F treatment confirms that zebrafish Abcb6 is not a glycoprotein, unlike its human homolog (N = 1 as experiment is a qualitative confirmation of sequencing data). Western blots show zebrafish Abcb6 binds ATP-agarose (c) and various porphyrins like its human homolog (d). Representative data shown, N = 3 biological replicates for each pulldown, with each experiment repeated from transfection to western blot. e Overlay of a representative structure from the MD simulations of the zebrafish Abcb6 (green) and ABCB6 WT (indigo/turquoise as shown in Fig. 2) showing that the two proteins adopt a similar conformation. f Overlay of zebrafish Abcb6 WT and zebrafish Abcb6 L356P showing the change in conformation of the coupling helix. Source data are provided in the Source Data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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