Fig. 8

Dynamic recruitment of Rasip1 through Heg1 and Krit1 and its response to contractility.

A Diagram showing the interactions between Rasip1, Heg1, Krit1, and Cdh5. B ZO-1 and Rasip1 antibody staining in wild-type embryos, heg1^m552, krit1^t26458 and cdh5^ubs8 mutants at 30 hpf. White arrows label the initial cell-cell contact sites between tip cells with enriched Rasip1. White arrowheads mark the apical compartments with enriched Rasip1. C ZO-1 and Rasip1 antibody staining in wild-type embryos, heg1^m552, krit1^t26458 and cdh5^ubs8 mutants at 32 hpf. White arrowheads indicate Rasip1 enrichment along the junctions. D Violin plot showing the Rasip1 boundary-to-apical ratio of average intensity per ring in wild-type embryos (n = 49 cells from 6 embryos), heg1^m552(n = 33 cells from 7 embryos), krit1^t26458 (n = 57 cells from 7 embryos), and cdh5^ubs8 mutants (n = 47 cells from 8 embryos). Data are presented as mean ± SD. ***P = 0.0002, *P = 0.0492, n.s., not significant, 2-tailed unpaired t-test. E Time-lapse images of Cdh5-Venus and Rasip1-Scarlet-I in krit1 mutants showing poor enrichment of Rasip1-scarlet-I along the junctions. F, G Activation of Opto-RhoA induced Rasip1-Scarlet-I enrichment at the apical compartment (F) and junctions (G). White arrowheads and arrows indicate the colocalized Rasip1-Scarlet-I and Opto-RhoA-Cherry clusters at the apical compartment and junctions, respectively. All scale bars: 10 μm. Source data are provided as a Source Data file.