Fig. 3

Adult sec31anju221 escapers display overt glycogenic hepatopathy. (A) Representative Kaplan?Meier plot for sec31anju221 fish and clutchmates from one of three independent experiments. n=80 for each group; P<0.0001 (Mantel?Cox test). (B) Live images of control and sec31anju221 zebrafish at 60 dpf. The livers are outlined with dashed lines. Lateral view, anterior to the left. Scale bars: 1 mm (left); 1 mm (right). (C) Ratio of the liver to body mass from 60 dpf sec31anju221 fish and clutchmates. n=11 for each genotype. (D) Representative photographs of H&E and ORO staining. Arrowheads indicate oil droplets. Scale bars: 20 ?m. (E) Electron micrographic pictures of liver biopsy from control and sec31anju221 zebrafish. The distribution of intracellular glycogen granules is outlined with dashed lines. ER, endoplasmic reticulum; GG, glycogen granule; M, mitochondria; N, nucleus. The inset shows an enlarged view of the boxed area. Images are representative of three fish per group. Scale bars: 2 ?m. (F) Representative photographs of PAS staining of liver and muscle sections. Images are representative of six fish per group. Scale bar: 50 ?m. (G) Triglyceride (n=8), cholesterol (n=6) and glycogen (n=8) levels in fish livers were measured with enzymatic colorimetric assays. Each data point represents an individual animal with bars representing mean±s.e.m. Comparison between groups was performed using unpaired two-tailed Student's t-test. *P<0.05; **P<0.01; ****P<0.0001.