Fig. 3

The PI3K/AKT signaling pathway is suppressed in the myd88?/? endocardium.a, Experimental plan for bulk RNA-seq analysis on sorted endocardial cells from ET(krt4:EGFP); myd88+/+ and ET(krt4:EGFP); myd88?/? ventricles at 96 hpci. b, Heatmap showing differential expression of significantly (P < 0.05) downregulated genes in the myd88?/? endocardium at 96 hpci. c, Representative images of immunostaining for EGFP (endocardial cells, blue), Fli1 (endothelial cell nuclei, magenta) and pAkt (phosphoAkt, green) on sections of cryoinjured ET(krt4:EGFP); myd88+/+ and ET(krt4:EGFP); myd88?/? ventricles at 96 hpci. d, krt4:EGFP+Fli1+pAkt+/krt4:EGFP+Fli1+ cell percentage in myd88+/+ and myd88?/? 50-?m-wide areas on the basal-most side of the injured tissue at 96 hpci. The dots in the graph represent individual ventricles; data are shown as the mean ± s.d.; n = 8 myd88+/+ and n = 5 myd88?/?. Statistical test: Student?s t-test. e, Representative images of immunostaining for EGFP (endocardial cells, blue), Fli1 (endothelial cell nuclei, magenta) and PCNA (proliferation marker, green) on sections of cryoinjured ET(krt4:EGFP); myd88+/+ and ET(krt4:EGFP); myd88?/? ventricles at 96 hpci. f, krt4:EGFP+Fli1+PCNA+/krt4:EGFP+Fli1+ cell percentage in myd88+/+ and myd88?/? 50-?m-wide areas on the basal-most side of the injured tissue at 96 hpci. The dots in the graph represent individual ventricles; data are shown as the mean ± s.d.; n = 7 myd88+/+ and n = 5 myd88?/?. Statistical test: Student?s t-test. The yellow dashed lines delineate the injured area; the yellow arrowheads point to krt4:EGFP+Fli1+pAkt+ (c) and krt4:EGFP+Fli1+PCNA+ (e) cells; the red arrowheads (c) point to krt4:EGFP+Fli1+pAkt? cells. Scale bars, 100 ?m.