FIGURE

Fig. 4

ID

ZDB-FIG-240903-67

Publication

Harboe et al., 2024 - The metalloproteinase PAPP-A is required for IGF-dependent chondrocyte differentiation and organization

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Fig. 4

Normal morphology of Meckel?s cartilage depends on IGF signaling. (A,B) Representative images of Alcian blue-stained wild-type zebrafish larvae treated with the IGF-IR inhibitor NVP-AEW541 (4 �M) or control solution (DMSO in E3) from 3 to 4 dpf. A magnification of one side of Meckel?s cartilage and a schematic outline of the chondrocytes in Meckel?s cartilage are also shown. (C) Length of Meckel?s cartilage after control (n = 8) and NVP-AEW541 (n = 8) treatment. Data are from two independent experiments. (D?F) A similar experiment using the structurally distinct IGF-IR inhibitor BMS-75807 (0.45 �M, n = 9; control group, n = 9). Data are from three independent experiments. Results are shown as mean � SD, and are analyzed by Student?s t-test. ****p < 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Conditions:	chemical treatment by environment: BMS-754807 chemical treatment by environment: insulin-like growth factor receptor 1 antagonist
Observed In:	Meckel's cartilage
Stage:	Day 4

Phenotype Detail

Acknowledgments

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