Fig. 3

R-loop landscapes during parental-to-zygotic transition. A. Schematic of the quantification method used in ULI-ssDRIP-seq. B. Schematic of early developmental stages examined in this study, including sperm, oocyte, 4-cell, 256-cell, dome stages and dorsal muscle (marked with red circle) from adult fish. Hours post fertilization, hpf. C. Relative levels of total R-loops in the nuclear genome from two biological replications of oocyte, sperm, 4-cell, 256-cell, dome, and muscle groups respectively. D. IGV snapshots of wR-loop and cR-loop signals from oocytes, sperms, 4-cell, 256-cell, dome embryos, and muscles (T1). E. Percentages of ULI-ssDRIP-seq reads from two biological replications of oocyte, sperm, 4-cell, 256-cell, dome, and muscle groups, mapped to the mitochondrial, 45S rRNA genes, or other genomic regions. F. Percentages of ULI-ssDRIP-seq reads from two biological replications of oocyte, sperm, 4-cell, 256-cell, dome, and muscle groups, mapped to maternal-type or somatic-type 45S rRNA genes.