FIGURE

Fig. 6

ID

ZDB-FIG-240708-45

Publication

McKaige et al., 2024 - Mitochondrial abnormalities contribute to muscle weakness in a Dnajb6 deficient zebrafish model

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Fig. 6

Western blot analysis shows dnajb6 double mutants have an increase in Pink1 levels indicating mitochondrial damage. (A) VDAC protein levels were increased at 6 dpf in dnajb6b mutants and double mutants. (B) At 3 mpf VDAC protein levels were significantly increased in dnajb6a single mutants but not in double mutants. (C) Western blot of Pink1 at 6 dpf shows no significant differences between genotypes. (D) Double mutants show significantly higher levels of Pink1 compared to their wildtype siblings at 3 mpf. Values are normalised to wildtype and GAPDH was used as a loading control. Error bars represent SEM for three biological replicates at 6 dpf and eight samples from 3 biological replicates at 3 mpf. Analysis was completed using a one-way ANOVA and two-way Dunnett multiple comparisons test.

Expression Data

Antibodies:	Ab2-pink1 Ab3-vdac1
Fish:	TU dnajb6a^mnu13/mnu13 (TU) dnajb6b^mnu14/mnu14 (TU) dnajb6a^mnu13/mnu13; dnajb6b^mnu14/mnu14 (TU)
Anatomical Terms:	muscle whole organism
Stage Range:	Day 6 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dnajb6a^mnu13/mnu13 (TU) dnajb6b^mnu14/mnu14 (TU) dnajb6a^mnu13/mnu13; dnajb6b^mnu14/mnu14 (TU)
Observed In:	muscle whole organism
Stage Range:	Day 6 to Adult

Phenotype Detail

Acknowledgments

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