FIGURE

Fig. 3

ID

ZDB-FIG-240627-20

Publication

Kaiyrzhanov et al., 2024 - Clinical and Molecular Spectrum of Autosomal Recessive CA8-Related Cerebellar Ataxia

Other Figures

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Fig. 3

CRISPR/Cas9 dre-ca8 F0 knockout impairs early brain development. (A) Lateral view of 24 hpf zebrafish embryos (midbrain-hindbrain boundary indicated by purple arrowheads) and (B) quantification demonstrating altered development of the forebrain, midbrain, and hindbrain in ca8 crispant embryos. (C) ca8-crispants also present marked abnormal brain ventricle formation at 24 hpf. (D) Schematic illustration of the normal morphology of zebrafish brain ventricles at 24 hpf. (E) Quantification of abnormal brain ventricle formation observed at 24 hpf in dre-ca8 crispant embryos. Significance determined by Mann-Whitney tests. Scale bar, 250 μm. **P < 0.01, ***P < 0.001, ***P < 0.0001. F, forebrain; M, midbrain; H, hindbrain; cb, cerebellum, see Pose-Méndez et al;16 dpf, days post fertilization; hpf, hours post fertilization [Color figure can be viewed at wileyonlinelibrary.com]

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TU + CRISPR5-ca8 + CRISPR6-ca8 + CRISPR7-ca8 + CRISPR8-ca8
Knockdown Reagents:	CRISPR5-ca8 CRISPR6-ca8 CRISPR7-ca8 CRISPR8-ca8
Observed In:	brain forebrain ventricle fourth ventricle midbrain hindbrain boundary tectal ventricle
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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