Figure 1

Loss of primary and motile cilia in the elipsa mutant larval brain

(A1–A4 and B1–B4) Staining of dissected 4 dpf brains with arl13b antibody to stain all cilia in the brain, n = 8 controls and 9 mutants. A1 At 4 dpf, arl13b stained cilia were located all over the brain, represented in insets drawn in the telencephalon (A2), optic tectum (A3) and brainstem (A4).

(B1) In the elipsa mutants, arl13b-expressing cilia were absent in the entire brain, as represented by insets drawn in the telencephalon (B2), optic tectum (B3), and brain stem (B4). (C1–C4 and D1–D4) Staining of dissected 4 dpf brains with glutamylated tubulin used to identify motile cilia, n = 5.

(C1) At 4 dpf, single glutamylated tubulin-positive cilia were present in the forebrain choroid plexus (C2), on the dorsal roof and ventral part (C3) of the tectal/diencephalic ventricle and in the rhombencephalon choroid plexus (C4).

(D1) In elipsa mutant, glutamylated tubulin-positive cilia were absent in the telencephalon (D2), optic tectum (D3), and brain stem (D4). Tel, Telencephalon; Teo, Optic Tectum; CCe, Corpus Cerebelli; BS, Brain stem. Cilia loss indicated by # symbol and nonspecific signal from glutamylated tubulin is represented by ∗ symbol.

See also Figures S1 and S2.