Fig 4

gsx1 mutants have disrupted prey capture and normal light-mediated turn bias.

(A- Aⁱⁱⁱ) Experimental setup for prey capture assay; eight 7 dpf larvae per 6 cm dish, except in controls (no larvae). (B) Bar graph depicts rotifer/mL average across each group with individual data points showing all trials color coded (cyan, black, and orange) (n = 3) for controls (n = 7 dishes), wildtypes (n = 9 dishes), and gsx1^y689 (n = 9 dishes); pre-feeding (dark grey bars), post-feeding (light grey bars). Power analysis revealed 100% for each condition, p<0.05. gsx1^+/+ post count of rotifers/mL were statistically different from controls and gsx1^y689, (F(2,24) = [3.44], p<0.001). Error bars displayed as ±SEM. No significant differences found between control and gsx1^y689 post-feeding count analysis, p = 0.63. (C-F) Motor features extracted from the same pool of behavior tested larvae (n = 53 gsx1⁺ light grey bars, n = 65 gsx1^y689/+ yellow bars, n = 21 gsx1^y689 dark grey bars. Data collected from four independent clutches. (C) Schematic of recording field of view for turn bias assay. Four individual larvae recorded simultaneously. Not to scale (top). Light series during recordings showing light on (yellow) and light off (black) intervals. Scale bar represents a 30 second time period (middle). Representative traces during light on and off periods (bottom). (D) Match index during light on and dark trials (noted on the y-axis). Circle asterisk denotes p < 0.05 using a one-way Wilcoxon Signed Rank test to 0.5 representing random turn bias (shown with dotted red line). (E-F) Total turn angle and fractal dimension, representing other dark response motor features. * shows T test between light and dark responses for each genotype. Black circles show individual larva datapoints. Error bars displayed as ±SEM.