Fig. 3

JNK signaling dynamics during spinal cord development. (A) Cellular localization changes of cJun-NES-Venus fluorescence during development. Images shown are from the same individual fish over time. Left: Maximum intensity projection of cJun-NES-Venus fluorescence. Right: Heatmap of calculated JNK-KTR values in each individual cell. Scale bar: 100 µm. (B) JNK-KTR indicates high mean JNK activity among all cells at 48 and 72 hpf in the larval spinal cord and lower mean activity at 96-144 hpf (n=7-9 animals across three experiments; Welch's ANOVA with Dunnett's T3 multiple comparison test). (C) The proportion of cells with high JNK activity follows the same trend as mean JNK activity, with more cells with high JNK at 48 and 72 hpf than at 96-144 hpf (n=7-9 animals across three experiments; Welch's ANOVA with Dunnett's T3 multiple comparison test). (D) JNK activity shows no bias on the anteroposterior axis of the developing spinal cord at 96 hpf. The dashed black line represents a linear line of fit (R2 shown bottom right). Colored line represents the mean JNK activity in each bin (n=9 animals across three experiments; error bars represent s.e.m.; one-way Welch's ANOVA). (E,F) JNK activity shows a dorsal bias in JNK activity at 72 and 96 hpf. In addition to the peak of JNK activity at the dorsal side of the tissue, a local maximum can be identified more ventrally, potentially indicating multiple foci of JNK activity within the developing spinal cord. Dashed black line represents a cuboidal line of fit (R2 shown bottom right). Colored line represents the mean JNK activity in each bin (n=7-9 animals across three experiments; error bars represent s.e.m.; one-way Welch's ANOVA). a.u. arbitrary units.