Fig. S6

Cryo-EM maps and models of codon-anticodon interactions on the ribosome complexes, related to Figure 6 (A and B) Cryo-EM structures of codon-anticodon interactions at the A-site of human 80S ribosomes bound by human tRNAAsp bearing manQ34 (green) and GAU codon (magenta) (PDB: 8JDK ) (A), and human tRNATyr bearing galQ34 (green) and UAU codon (magenta) (PDB: 8JDM ) (B). 18S rRNA is colored cyan. H-bonds are shown as dotted lines. (C–F) Cryo-EM maps and models show base pairing between the 3rd position of the codon and the wobble position of the anticodon at the P- and A-sites of human 80S ribosomes. Hydrogen bonds are depicted as dotted lines. Unpostprocessed and postprocessed maps are depicted in purple surface and gray mesh, respectively. (C) PDB: 8JDJ with the unpostprocessed map at level 0.014, and the postprocessed map at level 0.02 (EMDB: EMD-36178). (D) PDB: 8JDK with the unpostprocessed map at level 0.0129, and the postprocessed map at level 0.018 (EMDB: EMD-36179). (E) PDB: 8JDL with the unpostprocessed map at level 0.0109, and the postprocessed map at level 0.018 (EMDB: EMD-36180). (F) PDB: 8JDM with the unpostprocessed map at level 0.012, and the postprocessed map at level 0.018 (EMDB: EMD-35161). (G–L) Cryo-EM maps and models show base pairing between the 3rd position of the codon and the wobble position of the anticodon at the A-site of E. coli 70S ribosomes. Hydrogen bonds are depicted as dotted lines. Unpostprocessed and postprocessed maps are depicted in purple surface and gray mesh, respectively. (G) PDB: 7Y7C with the unpostprocessed map at level 0.0071, and the postprocessed map at level 0.0219 (EMDB: EMD-33660). (H) PDB: 7Y7D with the unpostprocessed map at level 0.0058, and the postprocessed map at level 0.0225 (EMDB: EMD-33661). (I) PDB: 7Y7E with the unpostprocessed map at level 0.0075, and the postprocessed map at level 0.0298 (EMDB: EMD-33662). (J) PDB: 7Y7F with the unpostprocessed map at level 0.0075, and the postprocessed map at level 0.0278 (EMDB: EMD-33663). (K) PDB: 7Y7G with the unpostprocessed map at level 0.0072, and the postprocessed map at level 0.0308 (EMDB: EMD-33664). (L) PDB: 7Y7H with the unpostprocessed map at level 0.0075, and the postprocessed map at level 0.0255 (EMDB: EMD-33665).