FIGURE

Fig. 1

ID

ZDB-FIG-231228-140

Publication

Torraca et al., 2023 - Zebrafish null mutants of Sept6 and Sept15 are viable but susceptible to Shigella infection

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Generation of sept6 and sept15 mutants. (a) Schematic representation of the general septin protein structure and consequence of gene mutations at the level of the protein structure. (b,c) Specific representation of Sept6 (b) and Sept15 (c) protein structure and consequence of gene mutations at protein levels in Sept6 and Sept15 mutants. A sept6 null allele was generated by CRISPR/Cas9 gene editing. A sept15 null allele was identified from an ENU mutagenesis screen. (d,e) Genotyping of the adult offspring of sept6 (d) and sept15 (e) heterozygote carriers indicated no inheritance disequilibrium and homozygote mutants reached adulthood at the same rate as co-housed wild-type fish. The proportions of the different genotypes did not significantly differ from the attended Mendelian proportions by chi-squared test. Sample size: a total of 48 (sept6) and 41 (sept15) adults (~6 months old) were genotyped

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	septin6^ls01/ls01 (AB) septin15^{sa44249/sa44249} (AB/TL) septin6^ls01/+ (AB) septin15^sa44249/+ (AB/TL)
Observed In:	whole organism
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cytoskeleton