FIGURE

Figure 2

ID

ZDB-FIG-231030-74

Publication

Toualbi et al., 2023 - Gene Augmentation of CHM Using Non-Viral Episomal Vectors in Models of Choroideremia

Other Figures

All Figure Page

Back to All Figure Page

Figure 2

Rescue of CHM patient-derived fibroblasts. (A) Human dermal fibroblasts (HDF) from a CHM patient with the c.126C>G (p.Y42*) mutation were transfected with pS/MAR-CAG-CHM (V2). GFP expression was detected at 48 h post-transfection and was maintained at 120 h post-FACS. (B) Using Western blot, REP1 protein was detected in the transfected CHM patient fibroblasts at 11 days and 35 days post-FACS. REP1 was detected in wild-type control fibroblasts but was almost completely absent in non-transfected CHM fibroblasts. (C) As a measure of prenylation function, an in vitro prenylation assay was performed to detect the size of the unprenylated Rab protein pool in transfected versus non-transfected CHM fibroblasts at 7 days post-transfection. A significant decrease in the presence of unprenylated Rabs was observed in the transfected fibroblasts. * p < 0.05. (ns = non significant).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.