FIGURE

Fig. 2

ID

ZDB-FIG-231025-1

Publication

Boos et al., 2023 - Hdac1-deficiency affects the cell cycle axis Cdc25-Cdk1 causing impaired G2/M phase progression and reduced cardiomyocyte proliferation in zebrafish

Other Figures

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Fig. 2

Hdac1-deficiency leads to an activation of DNA damage response (A) Dotplot displays the top 15 activated and suppressed Gene Ontology terms. Dots are color-coded according to the adjusted p-value and their size is in relation with the counts. The gene ratio describes the ratio of counts and total number of genes in the gene set. The GO-Term “DNA damage checkpoint” is highlighted in red. (B) Western Blot analysis of wildtype sibling (wt) and baldrian (bal) protein lysate showing the amount of γH2AX with pan Cadherin as control. (C) Quantification of Western Blot analysis (bal 14.56 ± 14.86, n = 8, Mann-Whitney-test, ∗∗∗p < 0.001). (D–E) Merged images of embryonic hearts of wt (D) and bal (E) from bal x Tg[myl7:DsRed-NLS] expressing DsRed in cardiomyocyte nuclei (red) stained with γH2AX antibody (green) (v: ventricle, a: atrium, scale bar 20 μm). (F) Quantification of γH2AX⁺ cardiomyocytes in relation to total cardiomyocyte numbers (wt 25.01 ± 8.749%, n = 7, bal 62.66 ± 12.93%, n = 8, Mann-Whitney-test, ∗∗∗p < 0.001).

Expression Data

Gene:	DsRed2
Antibodies:	Ab4-pan-cadherin Ab7-h2afx
Fish:	WT hdac1^{ulm107/ulm107} f2Tg hdac1^{ulm107/ulm107}; f2Tg
Anatomical Terms:	cardiac muscle cell whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hdac1^{ulm107/ulm107} hdac1^{ulm107/ulm107}; f2Tg
Observed In:	cardiac muscle cell whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biochem. Biophys. Res. Commun.