FIGURE

Figure 2

ID

ZDB-FIG-231019-4

Publication

Ott et al., 2023 - A novel role for the chloride intracellular channel protein Clic5 in ciliary function

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Figure 2

Subcellular localization analyses of Clic5 in the pronephric tubule of zebrafish. (A–D) Triple whole-mount immunostaining on 1dpf old Tg(actb2:Mmu.Arl13b-GFP) zebrafish embryos using anti-GFP (labeling Arl13b-GFP, i.e. the ciliary axoneme) (A), anti-γTubulin (labeling the ciliary basal body) (B) and anti-Clic5 (monoclonal antibody produced in mouse) (C) antibodies reveals ciliary localization of Clic5 in the pronephric tubule. (D) Image is (A–C) merged. (E) 3D reconstruction and segmentation of Arl13b-GFP (green), γTubulin (blue) and Clic5 (gold) using Imaris software. Clic5 voxels co-localize with those of ciliary basal body (white arrows) and ciliary axoneme voxels (yellow arrows), respectively. Scale bar, 5 µm.

Expression Data

Antibody:	Ab1-clic
Fish:	hsc5Tg
Anatomical Terms:	pronephric tubule axoneme pronephric tubule ciliary basal body pronephric tubule cilium
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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