FIGURE

Figure 2.

ID

ZDB-FIG-231002-64

Publication

Gao et al., 2023 - Sensory deficit screen identifies nsf mutation that differentially affects SNARE recycling and quality control

Other Figures

All Figure Page

Back to All Figure Page

Figure 2.

The I209N mutation in Nsfa selectively affects hearing and vestibular function without causing paralysis

(A) Still frames from high-speed videos of larvae. nsfa^st53 homozygotes were paralyzed and unresponsive to touch, whereas nsfa^I209N homozygotic and nsfa^I209N/st53 larvae displayed robust startle reflexes.

(B) (B and C) Acoustic evoked behavioral responses of larvae exposed to a 600-Hz stimulus at the intensities indicated normalized to homozygous WT siblings. n indicates number of fish tested.

(D–K) Vestibulospinal reflexes are reduced in nsfa^I209N homozygotic and nsfa^I209N/st53 larvae. (E–G) Raw traces (blue) of the tail movements of representative WT and mutant larvae. The rolling median (orange trace) is a movement artifact. (H–K) The maximum tail angle (H and I) and the normalized integral (J and K) of sibling cohorts were quantified using ZebraZoom. n indicates number of fish tested.

Mean ± SEM and two-way ANOVA with Benjamini-Hochberg correction for each dataset were performed. *p < 0.05, **p < 0.01, ***p < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nsfa^st53/st53 nsfa^{zf3929/zf3929} nsfa^st53/+ nsfa^zf3929/+ nsfa^st53/+; nsfa^zf3929/+
Observed In:	hair cell synaptic vesicle mechanosensory behavior motor behavior vestibular reflex
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.