Fig. 2

Effects of the Nrf2 mutation on the phenotypes of keap1a;keap1b-DKO zebrafish. (A) Survival rates of offspring from Ka0Kb1N1 parents at 7, 14, 21, and 28 dpf. The red, pink, and lavenderblush bars represent Ka0Kb0N2, Ka0Kb0N1, and Ka0Kb0N0 larvae, respectively. N0, N1, and N2 designate homozygous, heterozygous, and wild-type of the Nrf2 mutant (nfe2l2a^fh318), respectively. (B) Survival rates of 3- to 6-mpf offspring from Ka0Kb1N1 parents. Data from 3 independent experiments were combined. (C) Eating analysis of offspring from Ka0Kb1N1 parents at 8 dpf. Data from 3 independent experiments were combined. (D) Survival rates of offspring from Ka1Kb0N1 parents without feeding. The survival was observed every 24 h from 6 to 11 dpf. The tested numbers were as follows: Ka0Kb0N2 n = 5, Ka0Kb0N1 n = 25, Ka0Kb0N0 n = 11. The asterisks indicate significant differences (**p < 0.01). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)