FIGURE

Fig. 1

ID

ZDB-FIG-230729-1

Publication

Lei et al., 2023 - Hyperhomocysteinemia potentiates megakaryocyte differentiation and thrombopoiesis via GH-PI3K-Akt axis

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Fig. 1

Hcy facilitates MKs differentiation and platelet production. A Peripheral PLT in C57BL/6J mice. Significance according to two-tailed unpaired t test (n = 8). B Representative images for staining and C quantification of mpl:eGFP⁺ cells (green) in zebrafish Tg(mpl:eGFP)smu4 larvae caudal hematopoietic tissue (CHT) region. Scale bars, 50 μm. Significance according to one-way ANOVA with Tukey multiple comparisons test (n = 10). D PLT recovery after platelet depletion by monoclonal rat anti-mouse CD42b antibody (Anti-CD42b MoAb). Significance according to two-tailed unpaired t test (n ≥ 5). E PLT in splenectomized mice. Significance according to two-tailed unpaired t test (n = 8). F PLT in C57BL/6J-Mpl^hlb219/J mice. Significance according to two-tailed unpaired t test (n ≥ 5). G Representative images for staining and H quantification of mpl:eGFP⁺ cells (green) at mpl-mutational zebrafish Tg(mpl:eGFP)smu4;mpl^smu3 larvae CHT region. Scale bars, 50 μm. Significance according to Welch ANOVA test with Dunnett T3 multiple comparisons test (n = 10). I 13 cell clusters were displayed by UMAP. Colors indicate cell types. CMP, common myeloid progenitor; GMP, granulocyte-monocyte progenitor; MEMP, megakaryocyte-erythroid-mast cell progenitor; MK, megakaryocyte. J Bar diagram showing the representative GO biological process terms of MKs subpopulations. K Bar diagram showing the percentage of each MKs subpopulation number to all cells. L Venn diagram visualizing the up-regulated biological processes of each MKs subpopulation in Hcy group compared with control group. M Representative images of PPF detected by phase contrast imaging (left) and confocal microscopy (right). β1-tubulin (green) and DAPI (blue) were stained; Scale bars, 20 μm. N Histogram showing the number of PPF-MKs. Significance according to two-tailed unpaired t test (n = 6). O Representative images and P quantification of CD41⁺ MKs (green) in mice femurs bone marrow by immunofluorescence staining. Scale bars, 50 μm. Significance according to two-tailed unpaired t test (n = 8). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns not significant

Expression Data

Gene:	EGFP
Fish:	sm9Tg
Condition:	chemical treatment by environment: homocysteine
Anatomical Term:	thrombocyte
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sm9Tg mpl^sm10/sm10; sm9Tg
Condition:	chemical treatment by environment: homocysteine
Observed In:	caudal hematopoietic tissue thrombocyte thrombocyte
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Hematol. Oncol.