Dendrites of neurons are reduced after Trappc9 loss-of-function. (A-B), RNA in situ hybridization and RT-qPCR analysis of map2 mRNA expression in 48 hpf zebrafish embryos. The expression of map2 was reduced in mutant (zTrappc9m/m) and morphant (MO) zebrafish embryos. Representative images from n=20-25 embryos/group in 5 experiments (A); n=3 experiments (B). (C-D), Western blot analysis of MAP2 in adult mouse cerebral cortex showing significant reduction in the integrated density of MAP2 protein bands normalized with GAPDH. n=3 mice/group. (E-F), MAP2 immunostaining of neonatal (P1) mouse cerebral cortex showing a significant decrease in the area ratio of MAP2 fluorescence. Representative images from n=3 mice/group (E); n=10 sections (F). (G-H), MAP2 immunostaining of P21 mouse cerebral cortex showing a significant decrease in the area ratio of MAP2 fluorescence. Representative images from n=3 mice/group (G); n=9 sections (H). (I-J), MAP2 immunostaining of adult mouse cerebral cortex showing a significant decrease in the area ratio of MAP2 fluorescence. Representative images from n=3 mice/group (I); n=8 sections (J). Data are means ± SEM; t-tests (F); One-way ANOVA (B, D, H, J); ****P≤0.0001; ***P≤0.001; Scale bars: 50 μm (A, E, G, I).
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