FIGURE

Fig. 3

ID

ZDB-FIG-230703-28

Publication

Fetsko et al., 2022 - Brain endothelial cells acquire blood-brain barrier properties in the absence of Vegf-dependent CNS angiogenesis

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Fig. 3

Fig. 3. Vegfr tyrosine kinase inhibitors, DMH4 and AV-951, block CNS angiogenesis but not barriergenesis. (A) Representative confocal images (dorsal views; anterior left) of untreated, DMSO-treated, or inhibitor-treated glut1b:mCherry, plvap:EGFP embryos. Embryos were treated at 24 hpf then imaged at 54 hpf. Note that the inhibitor-treated embryos lack CtAs but express glut1b:mCherry in the PHBCs indicating that barriergenesis occurs in the absence of CNS angiogenesis. In addition, 1 μM DMH4 was as effective as 5 μM DMH4 at blocking CNS angiogenesis. AV-951 (1 μM) showed similar effects as 5 μM DMH4. Scale bars are 100 μm (top left) and 50 μm (top right). (B) Quantification of the number of CtAs at 2 dpf for untreated, DMSO-treated, or inhibitor-treated embryos (n = 3). (C) Quantification of the normalized RFI of glut1b:mCherry in untreated, DMSO-treated, or inhibitor-treated embryos at 2 dpf (n = 3). Data for (B) and (C) are presented as means ± SEM (∗∗∗p < 0.001; ns = not significant).

Expression Data

Gene:	mCherry
Fish:	sj1Tg; sj3Tg
Conditions:	chemical treatment by environment: tivozanib chemical treatment by environment: DMH4
Anatomical Term:	primordial hindbrain channel
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sj1Tg; sj3Tg
Conditions:	chemical treatment by environment: tivozanib chemical treatment by environment: DMH4
Observed In:	central artery central nervous system angiogenesis primordial hindbrain channel establishment of endothelial blood-brain barrier
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 494, Fetsko, A.R., Sebo, D.J., Taylor, M.R., Brain endothelial cells acquire blood-brain barrier properties in the absence of Vegf-dependent CNS angiogenesis, 46-59, Copyright (2022) with permission from Elsevier. Full text @ Dev. Biol.