FIGURE

Figure 2

ID

ZDB-FIG-230629-9

Publication

Erickson et al., 2023 - Regionalized Protein Localization Domains in the Zebrafish Hair Cell Kinocilium

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Figure 2

Distributions of tubulin, Ankef1a, Odf3l2a, and Saxo2 in the hair cell kinocilium at 5–6 dpf. (A1–D1) Pixel intensity-encoded images of tagged alpha-tubulin, Ankef1a, Odf3l2a, and Saxo2 proteins in the kinocilia of lateral cristae hair cells. Brighter colours indicate higher fluorescence intensity. (A2–D2) Scatter plots of averaged mean grey values (log2) for fluorescence intensity from kinocilia in the lateral cristae of YFP-TUBA (n = 35 kinocilia from 8 individuals), Ankef1a-GFP (n = 23 from 8 individuals), Odf3l2a-GFP (n = 30 from 11 individuals), and Saxo2-mKate2 (n = 17 from 6 individuals). Position 0 µm is from the proximal region near the base of kinocilia and position 32 µm is the distal-most tip. (E–H) Pixel intensity-encoded images of neuromast kinocilia from YFP-TUBA, Ankef1a-GFP, Odf3l2a-GFP, and Saxo2-mKate2 expressing zebrafish larvae. Scale bars are 5 µm in panels (A1–D1,E–H).

Expression Data

Genes:	emGFP mKate2 YFP
Fish:	idc16Tg unb4Tg unb5Tg unb6Tg unb7Tg
Anatomical Terms:	lateral crista kinocilium neuromast hair cell kinocilium
Stage Range:	Day 5 to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	unb7Tg
Observed In:	lateral crista kinocilium
Stage Range:	Day 5 to Day 6

Phenotype Detail

Acknowledgments

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