Fig. 4

(A) Experimental timeline to assess the rates of neuronal differentiation and progenitor self-renewal. Animal numbers are indicated for each genotype, and two independent replicates are shown.

(B) Immunohistochemistry for EdU (magenta), Sox2 (green), and HuC/D (blue) in SC sections of mstnb^+/+ and mstnb^−/− at 14 dpi. Regions in the rectangular boxes are shown at high magnification. Arrowheads indicate HuC/D^>+ EdU^>+ neurons or Sox2⁺ EdU^>+ progenitors.

(C) Regenerated HuC/D^>+ EdU^>+ neurons were quantified in D SC sections at 14 dpi. The proportions of HuC/D^>+ EdU^>+ neurons (%) representthe numbers of HuC/D⁺ EdU⁺ neurons normalized to the total numbers of nuclei for each section.

(D) HuC/D^>+ neurons were quantified in D SC sections at 14 dpi. The proportions of HuC/D^>+ neurons (%) represent the numbers of HuC/D^>+ neurons normalized to the total numbers of nuclei for each section.

(E) Sox2⁺ EdU^>+ progenitors were quantified in D SC sections at 14 dpi. The proportions of Sox2⁺ EdU^>+ progenitors (%) represent the numbers of Sox2⁺ EdU^>+ progenitors normalized to the total numbers of nuclei for each section.

(F) Sox2⁺ progenitors were quantified in D SC sections at 14 dpi. The proportions of Sox2⁺ progenitors represent the numbers of Sox2⁺ progenitors normalized to the total numbers of nuclei for each section.

Error bars depict SEM, and statistical significance was determined by two-way ANOVA. ANOVA p values and multiple-comparisons p values are indicated. *p < 0.05; ns, p > 0.05. Scale bars, 50 μm.