FIGURE

Figure 5

ID
ZDB-FIG-230228-130
Publication
Chopra et al., 2023 - Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Figure 5

HyPer ratios in WT animals and nox mutants

WT animals and nox mutants were generated in a Tg(ubb:HyPer)umc400 transgenic background, which permits in vivo assessment of ROS levels. (A) ROS Hyper ratios in individual WT animals (n = 8) observed at three time points during the day over three days post-amputation reveal an oscillatory pattern of ROS levels.

(B) ROS levels over three consecutive days post-amputation were significantly higher in the mornings (7a.m.) than in the afternoons (3 p.m.) or nights (10p.m.).

(C and D) Long-Scargle periodogram reveals that the ROS oscillations follow a daytime-dependent trend, with a periodicity of around 0.4 (∗ on graph), indicative of a ∼24 h circadian cycle (D).

(E) Graph of ROS levels measured at the same time of day (3p.m.) in WT (unamputated), WT (amputated), cybaumc403, duoxsa9892, and nox5umc402 animals.

(F) Graph of mean Hyper ratios measured over three consecutive days at 3p.m. reveals significantly attenuated post-amputation ROS levels among the duoxsa9892 mutants relative to the WT amputated fins and the other nox mutants. Indeed, ROS levels in the duoxsa9892 fins are significantly lower than even those found in unamputated WT fins. ROS levels in the amputated cyba mutant fins are significantly lower than those present in WT amputated fins but not as low as in the duoxsa9892 fins. ROS levels in the amputated nox5umc402 mutant fins are not significantly different from those present in amputated WT fins. (Bonferroni’s multiple comparisons test, ∗p < 0.5, ∗∗p < 0.01, ∗∗∗∗p < 0.0001). For A-D, WT (n = 8). For E-F, WT unamputated (n = 7); WT amputated (n = 8); cybaumc403 (n = 10); duoxsa9892 (n = 7); nox5umc402 (n = 7).

See also Figures S10 and S11.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ iScience