FIGURE
Fig. 4
- ID
- ZDB-FIG-230205-14
- Publication
- Lin et al., 2021 - Follistatin-controlled activin-HNF4α-coagulation factor axis in liver progenitor cells determines outcome of acute liver failure
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Fig. 4
Activin controls coagulation factor expression in LPCs through the SMADs-FOXH1 complex-controlled HNF4α. (A) Quantitative PCR and western blot measure mRNA and protein expression of HNF4α, F2, and F5 in HepaRG and BMOL cells with or without SMAD2/3 knockdown by RNAi. (B) ChIP assay was performed to examine the role of activin and SB431642 on the binding of SMAD2/3 and SMAD4 to the HNF4A gene promoters in indicated LPCs. (C) Quantitative PCR and western blot measure mRNA and protein expression of HNF4α, F2, and F5 in HepaRG and BMOL cells with or without FOXH1 knockdown by RNAi. (D) ChIP assay examined the role of activin and SB431642 in the binding of FOXH1 to the HNF4A gene promoters in HepaRG cells. (E) Co-IP was performed to measure SMADs-FOXH1 complex formation in HepaRG and BMOL cells. The expression of PPIA was used as loading control in ChIP assay. Tubulin was used as loading control in western blotting. *p < 0.05, **p < 0.01, and ***p < 0.001
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Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
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