Figure 6

Expression pattern of ZBEDX transcripts during the early developmental stages of zebrafish embryos. Embryos at different stages, as indicated, were collected and hybridized with antisense probe against ZBEDX mRNA using whole-mount in situ hybridization. Panels (A,C,E,H,K,N) are lateral views, while panels (B,D,F,I,J,L,M,O,P) are dorsal views. Panels (E,H,K,N) are lateral views, and the anterior of embryos was placed at the left, while panels (G,J,M,P) are dorsal views, and the anterior of embryos is on the top. At 12 hpf, ZBEDX transcripts were expressed in whole embryo. ZBEDX transcripts were initially apparent at the 1- (A,B) and 2-cell (C,D) stages. At 24–72 hpf, ZBEDX transcripts were expressed in forebrain (f), midbrain (m), hindbrain (h), retina (r), spinal cord (sc), and branchial arches (ba) (E–M). At 96-hpf, ZBEDX transcripts were expressed in midbrain (m) and hindbrain (h) (N–P).