FIGURE

Figure 8

ID

ZDB-FIG-230122-73

Publication

Shi et al., 2023 - Single-cell transcriptomic profiling of the zebrafish inner ear reveals molecularly distinct hair cell and supporting cell subtypes

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Figure 8

Zebrafish cabp2b+domain shares features with the mouse striolar region.

(A) Feature plot shows enrichment for the striola marker pvalb9 in cabp2b-expressing striolar cells. (B–D) HCR in situs in 5 dpf myo6b:GFP fish shows pvalb9 and cabp2b co-expression in the utricle. Scale bar = 10 μm. (E) Cartoon illustration of overlapping expression of pvalb9 (white) and cabp2b (magenta) that coincides with the line of hair cell polarity reversal. (F, G) Whole-mount RNAScope confocal images of adult zebrafish utricles showing expression of pvalb9 relative to (F) cabp1b (n=3) and (G) cabp2b (n=4). Scale bar = 25 μm. (H,I) Whole-mount RNAScope RNA and protein co-detection assay showing co-localization of cabp2b expression (RNA) and the hair cell line of polarity reversal indicated by Spectrin (protein) staining (n=3). Scale bar = 25 μm. Arrows denote hair cell polarity and dotted line outlines line of polarity reversal.

Expression Data

Genes:	cabp1b cabp2b pvalb9
Fish:	WT w186Tg
Anatomical Term:	utricle auditory receptor cell
Stage Range:	Day 5 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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