Fig. 4

Expression pattern of zebrafish stanniocalcin 1 (stc1), adenomatosis polyposis coli down-regulated 1-like (apcdd1l), cadherin 16/ksp cadherin (cdh16), frizzled related protein (frzb),convertase subtilisin/kexin type 2 (pcsk2) and prickle homolog 3 (prickle3) during zebrafish embryogensis.

(A) Zebrafish stc1 is expressed at 24 ​h post fertilization (hpf) at the end of the pronephros. With the development of the corpuscles of Stannius (CS), the expression is confined to the CS, where it is highly expressed during the first days of development. (B) Zebrafish apcdd1l co-localizes with the CS at 24 and 72 hpf. (C) Zebrafish cdh16 is expressed along most segments of the pronephros during early development, but primarily localizes with the corpuscles of Stannius (CS) at 48 hpf. (D) Zebrafish frzb is also expressed in several distal regions of the pronephros during early stages of development, but becomes confined to the CS at 48 and 72 hpf. (E) Zebrafish pcsk2 is prominently expressed in the corpuscles of Stannius (CS) at 48 hpf. A comparison of the expression levels derived from the single-cell RNA sequencing with the closely related furin a (pcsk3) and carboxypeptidase E (cpe) suggests a transient expression of cpe at 2 days post fertilization (dpf), followed by increased expression of pcsk2/3. (F) Zebrafish prickle3 co-localizes with the CS at 48 and 72 hpf.