Figure 7

Mutations in her9 enhance jag1b mutant phenotypes. (A) jag1b;her9 double heterozygotes were intercrossed and six days post fertilization (dpf) larvae were stained with Alcian Blue and Alizarin Red to label cartilage and bone. The individuals were then genotyped, the viscerocrania were dissected and flat mounted then imaged. The following craniofacial skeletal elements are indicated in the wild-type individual: opercle bone (op), branchiostegal ray (br), cleithrum (cl), foramen (f) palatoquadrate (pq). The dysmorphic foramen (df) and reduced posterior pq (rppq) phenotypes are indicated, and the small op bone phenotype is indicated by an arrowhead. Arrows mark the opercle mineralization defect associated with her9 homozygous mutants. Poorly stained posterior arch derived cartilages and one missing entopterygoid bone in jag1b-/-;her9+/- are staining and mounting artifacts in this individual and not representative phenotypes associated with this genotype. Scale bar is 50 μm (B) Genotyped preps from A were scored for penetrance of jag1b mutant-associated phenotypes. Asterisk in dysmorphic foramen row indicates that jag1b-/-;her9-/- is significantly different from both jag1b-/-;her9+/+ and jag1b-/-;her9+/- using a Fisher’s exact test with p<0.05. (C) Genotyped preps from A were scored for penetrance of her9 mutant-associated phenotypes.