FIGURE

Figure 5

ID

ZDB-FIG-221226-257

Publication

Weaver et al., 2022 - gldc Is Essential for Renal Progenitor Patterning during Kidney Development

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Figure 5

gldc morphant cloacae demonstrate increased levels of apoptosis and structural abnormalities. (A) Cell death assay of the cloaca in WT and gldc morphants at 24 hpf. Scale bar = 50 μM. (B) Quantification of AO⁺ cells in the cloaca; red arrowheads indicate example AO⁺ cells. (C) Live images of the cloacal region (outlined in red) in WT and gldc morphants at 24 hpf and 48 hpf. Scale bars = 50 μM. (D) Measurement of the cloacal area in WT and gldc MO. (E) WISH of mecom, pax2a, and gata3 in WT and gldc morphants at 24 hpf revealed enlarged cloacal regions. Scale bars = 100 μM. Data are mean ± s.d. AO⁺ cell counts and cloacal area were compared with unpaired T-tests.

Expression Data

Genes:	gata3 mecom pax2a
Fish:	WT WT + MO1-gldc + MO2-gldc
Knockdown Reagents:	MO1-gldc MO2-gldc
Anatomical Term:	cloaca
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-gldc + MO2-gldc
Knockdown Reagents:	MO1-gldc MO2-gldc
Observed In:	cloaca cloaca apoptotic process cloaca cell
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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