FIGURE

Fig. 2

ID

ZDB-FIG-221221-36

Publication

Ping et al., 2021 - Rapamycin relieves the cataract caused by ablation of Gja8b through stimulating autophagy in zebrafish

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Fig. 2

Ablation of Gja8b induces severe defects in organelle degradation of lens fiber cells. (A-C) Immunohistochemistry results showed that lens primary fiber cell markers (A, labeled by Zl-1 and Mip staining), endoplasmic reticulum (B, labeled by Kdel staining), cytoskeleton (C, labeled by F-actin staining), and nuclei (C, labeled by DAPI staining) remained in lens second fiber cells in gja8b mutants at 72 hpf. (D) The quantitative analysis of the average number of lens fiber cell nuclei shown in different gja8b mutants groups (WT-like group, minor group and major group) (mean ± SEM, n ≥ 30 zebrafish for each group, ****p < 0.0001). (E and F) Immunohistochemistry results showed that cytoskeleton (labeled by F-actin staining), and nuclei (labeled by DAPI staining) remained in the center of lens in gja8b mutants at 4 dpf (E) and 5 dpf (F). Scale bars: 50 μm

Expression Data

Antibodies:	Ab1-mip Ab14-hspa5 zl-1
Fish:	AB gja8b^{zko933a/zko933a}
Anatomical Term:	lens
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gja8b^{zko933a/zko933a}
Observed In:	lens lens central region lens fiber cell cytoskeleton lens fiber cell endoplasmic reticulum lens fiber cell endoplasmic reticulum disassembly lens fiber cell nucleus lens fiber cell organelle disassembly lens fiber cell differentiation
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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