FIGURE

Fig. 6

ID

ZDB-FIG-221209-25

Publication

Moss et al., 2021 - Autophagy coordinates chondrocyte development and early joint formation in zebrafish

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Fig. 6

atg13 mutation affects expression and production of key factors in cartilage development. (A) Left, confocal max projections of lower jaw at 5 dpf in wt and atg13‐mutant fish, immunostained for Sox9a (grey) and Col2a1 (red). Scale bars = 50 µm. Right, schematic showing regions of interest selected within lower jaw in modular image analysis program (SoxQuant). Colours correspond to graphs below. (B–D) Quantification of Sox9a expression measured as volume of Sox9a within Col2a1 positive cells from confocal z‐stack. Student's unpaired t test performed where ****p < .0001, ***p = .0007, *p = .0173; n = 12 for both. (E) Confocal max projections of the lower jaw at 7 dpf in Tg(atg13; Col2a1aBAC:mcherry) wt and atg13‐mutant larvae, immunostained for collagen Type X (ColX) (cyan) and mCherry (for Col2a1, red). Scale bars = 50 µm

Expression Data

Antibodies:	Ab1-col2a Ab2-mcherry Ab2-sox9a Ab3-col10a1
Fish:	WT atg13^{fci500/fci500} hu5910Tg atg13^{fci500/fci500}; hu5910Tg
Anatomical Terms:	Meckel's cartilage Meckel's cartilage hypertrophic chondrocyte ventral mandibular arch ventral mandibular arch hypertrophic chondrocyte
Stage Range:	Day 5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	atg13^{fci500/fci500} atg13^{fci500/fci500}; hu5910Tg
Observed In:	Meckel's cartilage Meckel's cartilage hypertrophic chondrocyte ventral mandibular arch ventral mandibular arch hypertrophic chondrocyte
Stage Range:	Day 5 to Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ FASEB J.