Fig. 4

(A and B) Relative expression levels of foxq2 and tbx2b in adult tissues (A), and 4-dpf larval anterior segments, posterior segments, and eyes (B). (C) Expression pattern of foxq2 and sws2 in 3-dpf and 5-dpf larvae examined by in situ HCR. The larval zebrafish are outlined with white dotted lines. d-v, dorsal-ventral axis; a-p, anterior-posterior axis. Scale bars, 100 μm. (D) Relative expression levels of tbx2b and foxq2 in the 5-dpf larval eyes of the six6a/six6b/six7 triple knockout (TKO). Means ± SEM (n = 5). *P < 0.05 by Student’s t test. Note that the six6a KO was used as the control because it shows normal levels of cone opsin gene expression as compared to the WT [see (20)]. The expression levels of sws2 and rh2 genes are reproduced from our previous paper (20). (E) Relative expression levels of tbx2b and foxq2 in the adult eyes of the six6a/six6b/six7 TKO. All of the fish used here are in the transgenic background, Tg(-3.5opn1sw2:EGFP)^kj11Tg, where EGFP is expressed in the SWS2 cone subtype. Data are represented by means ± SD (n = 3, WT; n = 2, six6a/six6b/six7 TKO). The expression levels of sws2 and rh2 genes are reproduced from our previous paper (20). (F) Relative expression levels of cone opsins and transcription factors responsible for photoreceptor gene expression in the eyes of the tbx2b (ja20) and foxq2 (ja74) mutants. Means ± SEM. *P < 0.05 by Student’s t test. The number of fish used was as follows: n = 5 (tbx2b WT), n = 4 (tbx2b mut); n = 5 (foxq2 WT), n = 5 (foxq2 mut). The expression levels of sws2 and rh2 genes in the foxq2 mutant and its control WT are reproduced from Fig. 1D.