Fig 4

Clutch size can be rescued by substitution of either cd9b mutant partner for an WT, however, fertilisation is only rescued when the cd9b KO male is substituted for an WT male.

Clutch size can be rescued by substitution of a cd9b mutant partner, male (m) or female (f), with an WT (A). A Kruskal-Wallis test with Dunn’s multiple comparisons test was carried out on the pooled data from three experimental repeats. n = minimum 12 pairs per cross. While clutch size can be partially or fully rescued by substitution of either cd9b mutant partner, the percentage of fertilised eggs appears to only return to WT levels upon substitution of a cd9b mutant male with an WT male (B). An ANOVA with Dunnett’s multiple comparisons test was carried out on arcsine transformed data pooled from three experiments. The average percentage of fertilised, dead and unfertilised eggs per clutch is shown in (C). n = minimum 10 pairs per cross for (B) and (C), except cd9b^is16 x cd9b^is16 where only 4 of 12 pairs laid. Significance of difference from WT control: **** p<0.001; *** p<0.005; ** p<0.01; * p<0.05; ns = not significant. Data was pooled from 3 experimental repeats carried out over several weeks.