Fig. 2

Nonsense mutations in pcsk9 do not affect zebrafish survival upon an S pneumoniae infection. S pneumoniae (4 700 000 CFU; SD 990 000 CFU) was injected into the abdominal cavity of (A) pcsk9^{tpu-13/tpu-13} (n = 21; 8 females, 13 males; survived: n = 16; 8 females, 8 males), pcsk9^tpu-13/+ (n = 24; 7 females, 17 males; survived: n = 17; 6 females, 11 males) and WT (pcsk9^tpu-13) (n = 26; 5 females, 21 males; survived: n = 15; 4 females, 11 males) as well as (B) pcsk9^{tpu-2,+15/tpu-2,+15} (n = 20; 4 females, 16 males; survived: n = 16; 3 females, 13 males), pcsk9^tpu-2,+15/+ (n = 54; 10 females, 44 males; survived: n = 49; 9 females, 40 males) and WT (pcsk9^tpu-2,+15) (n = 23; 12 females, 11 males; survived: n = 19; 10 females, 9 males) adult zebrafish, and their survival was followed for 7 days. Pneumococcus infected (240 CFU; SD 76 CFU) zebrafish larvae from both the (C) pcsk9^tpu-13 and (D) pcsk9^tpu-2,+15 background were followed until 5 dpi and their survival was recorded. Group sizes; pcsk9^{tpu-13/tpu-13} (n = 24; survived: n = 5), pcsk9^tpu-13/+ (n = 48; survived n = 19), WT (pcsk9^tpu-13) (n = 24; survived n = 4) and pcsk9^{tpu-2,+15/tpu-2,+15} (n = 17; survived n = 10), pcsk9^tpu-2,+15/+ (n = 24; survived n = 21), WT (pcsk9^tpu-2,+15) (n = 15; survived n = 13). The data were collected from single experiments. A log-rank (Mantel-Cox) test was used for statistics. dpi, days post infection