Fig. 5

The absence of macrophages led to the development of brain edema. (A) Phenotypes of edema-like symptoms at different time points after injury. Scale bar, 50 µm. (B) Statistics of edema volume in (A). (C) Statistics of edematous symptoms in Tg(coro1a:Dendra2—NTR) (C-D-N) and Tg(coro1a:GFP) (C–G) with DMSO/MTZ treatment. Purple arrowheads indicate the injury sites. (D) Analysis of zebrafish survival and edematous symptoms in Tg(coro1a:Dendra2-NTR) with DMSO/MTZ treatment after injury. (E) Survival rate of Tg(coro1a:Dendra2-NTR) with DMSO/MTZ treatment at 1 dpi. (F) The fluorescent images of HuC⁺ and TUNEL⁺ signals in edematous overflow. The purple dotted line represents the lateral edge of the edema and the yellow dotted line represents the internal measurement edge of the edema. Scale bar, 50 µm. (G) The fluorescent images of SOX2⁺ signals in edematous overflow. The red arrowheads indicate location of SOX2⁺ signals. Scale bar, 20 µm. (H) Detection of brain tissue by H&E staining of paraffin sections after injury. Red arrowheads indicate the injury site. Scale bar, 50 µm. (I) The bright field and fluorescent images of coro1a-Kaede⁺ cells and edema-like symptoms (purple dashed line). Scale bar, 50 µm. The purple arrowheads indicate the accumulation of macrophages at the wound site. (J) Statistics of edematous symptoms in cebpα mutant and sibling after injury. (K) Analysis of zebrafish survival and edematous symptoms in cebpα mutant and sibling after injury. (L) Survival rate of cebpα mutant and sibling at 1 dpi. (Data are shown as mean ± SEM. ns, no significance; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.)