FIGURE

Fig. 8

ID

ZDB-FIG-220926-8

Publication

Jussila et al., 2022 - Live imaging and conditional disruption of native PCP activity using endogenously tagged zebrafish sfGFP-Vangl2

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Fig. 8

FoxJ1a-lineage specific degradation of sfGFP-Vangl2 results in ectopic scospondin accumulation and disrupted Reissner fiber formation.

a, b Representative maximum intensity Z-stack projections of confocal micrographs, acquired through dorsally-oriented whole mount brains that were dissected from 21dpf vangl2^sfGFP/sfGFP;Tg(βactin2::loxP-mCherry-STOP-loxP-zGrad) control (a; n = 4) and vangl2^sfGFP/sfGFP;Tg(βactin2::loxP-mCherry-STOP-loxP-zGrad);Tg(foxj1a::iCre) mutant (b; n = 9) brains, and immunostained for polyglutamylated tubulin (magenta) and scospondin (green). Inverted, higher magnification images of scospondin immunostaining in forebrain (c, d) and rhombencephalic (e, f) ventricles of control (c, e) and mutant (d, f) brains, represented by boxed areas in a, b.

Expression Data

Antibody:	Ab1-RF
Fish:	vangl2^{hsc170Tg/hsc170Tg}; hsc105bTg/+; hsc185Tg/+ (TU)
Anatomical Terms:	forebrain ventricle fourth ventricle
Stage:	Days 21-29

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vangl2^{hsc170Tg/hsc170Tg}; hsc105bTg/+; hsc185Tg/+ (TU)
Observed In:	forebrain ventricle fourth ventricle Reissner's fiber
Stage:	Days 21-29

Phenotype Detail

Acknowledgments

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