FIGURE 8

Loss of Rnf2 leads to neuronal differentiation defects in zebrafish embryos. (A) Confocal images showing the expression of HuC/D and 5-HT in zebrafish brain at 96 hpf (40 × oil). Dorsal view. The white dashed boxes showing the overlapping of HuC/D⁺ and 5-HT⁺ signals in the brain of WT and rnf2^–/– embryos at 96 hpf. (B) Quantitation of percentage of 5-HT/HuC double positive cells in (A). The numbers of samples were 20 and 18 in 96 hpf WT and rnf2^–/– embryos, respectively. The star indicates significant differences at p ≤ 0.05. (C) Confocal images showing the expression of Mbp-GFP in zebrafish brain at 72 hpf (40 × oil). Dorsal view. The white dashed boxes showing the distribution of Mbp⁺ signals in the brain of WT and rnf2^–/– embryos at 72 hpf. (D) Quantitation of relative fluorescence intensity in (C). The numbers of samples were 28 and 20 in 72 hpf WT and rnf2^–/– embryos, respectively. The experiments were repeated three times. Scale bar = 0.1 mm.