FIGURE

Fig. 7

ID

ZDB-FIG-220905-39

Publication

Mattonet et al., 2022 - Endothelial versus pronephron fate decision is modulated by the transcription factors Cloche/Npas4l, Tal1, and Lmo2

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Fig. 7

Fig. 7. tal1 overexpression rescues endothelial development in npas4l mutants in an Etsrp-dependent manner.

(A) npas4l and tal1 mRNA injections rescue ISV formation in npas4l mutants. (B) Quantification of rescue: mRNA injections of npas4l and tal1, but not of etsrp or lmo2, rescue the ISV defects in npas4l^−/− embryos. Data are represented as individual data points, median, interquartile range, and extremes excluding potential outliers. P values were calculated by Poisson regression and adjusted for multiple comparisons between six groups using the Bonferroni correction. (C) tal1 mRNA injections do not rescue the vascular phenotype in etsrp^−/− embryos. Data are represented as individual data points, median, interquartile range, and extremes excluding outliers. P values were calculated by Poisson regression. (D and E) etsrp mRNA injections do not increase tal1 mRNA levels at the tailbud stage, whereas tal1 mRNA injections lead to increased etsrp mRNA levels at the same stage. Data are represented as individual data points as well as means ± SD. P values were calculated by unpaired two-sample t test and adjusted for multiple comparisons between five groups using the Bonferroni correction. (F) Uninjected and tal1 mRNA-injected npas4l^+/− and npas4l^−/− embryos. Ventrolateral npas4l reporter–expressing cells (arrowheads) are not present in tal1 mRNA-injected npas4l^−/− embryos, while the contribution of npas4l reporter–expressing cells to skeletal muscle (M) does not appear to be blocked. Scale bars, 500 μm (A) and 50 μm (F). P values were adjusted to counteract the multiple testing problem.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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